Application of both high-performance liquid chromatography combined with tandem mass spectrometry shotgun and 2-D polyacrylamide gel electrophoresis for streptococcal exoproteins gave reliable proteomic data

作者：Okamoto Akira; Hasegawa Tadao; Yamada Keiko; Ohta Michio^*

来源：Microbiology and Immunology, 2011, 55(2): 84-94.

DOI：10.1111/j.1348-0421.2010.00302.x

• 2-D PAGE
• LC-MS
• MS
• proteome analysis
• Streptococcus pyogenes

Streptococci secrete a large number of exoproteins including virulence-associated toxins and enzymes. To construct a reliable database of streptococcal exoproteins, we integrated the results that were derived from two approaches: LC-based shotgun proteomic analysis and 2-D PAGE-based proteomic analysis. We identified 74 and 82 proteins by LC-based and gel-based analysis, respectively. Forty-five proteins were identified by both methods. In addition, two proteins, one identified by both methods and the other only by LC-based shotgun analysis, were newly annotated. We therefore found the importance of combinational analysis by the two methods for the construction of a more reliable database.

• 出版日期2011-2