A thermally baffled device for highly stabilized convective PCR

作者：Chang Hsiao Fen Grace; Tsai Yun Long; Tsai Chuan Fu; Lin Ching Ko; Lee Pei Yu; Teng Ping Hua; Su Chen; Jeng Chien Chung^*

来源：Biotechnology Journal, 2012, 7(5): 662-666.

DOI：10.1002/biot.201100453

• Methods
• Rayleigh-Benard convective PCR
• Thermally baffled PCR instrument
• Yellow head virus

Rayleigh-Benard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small- diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low-cost, point-of-care device for PCR-based molecular diagnostics in the field.

• 出版日期2012-5
• 单位河北医科大学