Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)

作者：Maerz Anne; Moench Bettina; Roesch Petra; Kiehntopf Michael; Henkel Thomas; Popp Juergen^*

来源：Analytical and Bioanalytical Chemistry, 2011, 400(9): 2755-2761.

DOI：10.1007/s00216-011-4811-z

• SERS
• Microfluidic lab-on-a-chip
• Enzyme activity
• Thiopurine methyltransferase
• 6-Mercaptopurine

In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.

• 出版日期2011-7