Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei

作者：Bahr Luciana; Victoria Castelli Maria; Isabel Barolo Melisa; Ruiz Mostacero Nathalie; Elena Tosello Maria; Noeli Lopez Silvia

来源：Fungal Biology, 2016, 120(3): 424-432.

DOI：10.1016/j.funbio.2015.12.002

• Disease control
• Natural products
• Bioautography
• Cicer arietinum
• Inhibition

Ascochyta blight is the major disease attacking chickpea (Cicer arietinum) around the world. Since its first time report of isolation in Argentina in 2012, the pathogen has caused severe economic losses and has acquired a great importance. We report here the isolation of Ascochyta rabiei from infected chickpea beans cultivated in Santa Fe, Argentina; its identification by morphological analysis and molecular biology techniques based on internal transcribed spacer (ITS) sequence alignment, its biochemical characterization regarding the capacity to produce proteinase and phospholipase enzymes, and its antifungal susceptibility to common used antifungal agents. In order to detect new inhibitors for A. rabiei from natural sources, a bioautographic method was developed. From the screening method developed, we found that extracts from cultures of Aspergillus parasiticus are active against A. rabiei.

• 出版日期2016-3