A novel splitter design for microfluidic biochips using centrifugal driving forces

作者：Lin Samuel I En^*

来源：Microfluidics and Nanofluidics, 2010, 9(2-3): 523-532.

DOI：10.1007/s10404-010-0568-5

• CD-ELISA
• Microfluidic
• Splitter
• ELISA

Enzyme-linked immunosorbent assay (ELISA) is the most widely used immunoassay for the detection and quantification of biological agents (mainly proteins and polypeptides). One challenge in developing microfluidic devices for immunogenic and enzymatic assays is to split the fluid evenly into microchannels. To prevent uneven splitting, we propose a buffer-structure design, which holds the stream lines in the radial direction before they enter into bifurcation channels. To study the feasibility of this design, a three-dimensional numerical model, based on the Navier-Stoke equation, was constructed. Upon verification, it was successfully demonstrated that the proposed half-circle design can split flow evenly under the existence of Coriolis forces during operation.

• 出版日期2010-8