A comparison of high-throughput techniques for assaying circadian rhythms in plants

作者：Tindall Andrew J; Waller Jade; Greenwood Mark; Gould Peter D; Hartwell James; Hall Anthony^*

来源：Plant Methods, 2015, 11(1): 32.

DOI：10.1186/s13007-015-0071-9

• Luciferase
• Leaf movement
• Delayed fluorescence
• Infra-red gas exchange
• Circadian clock
• High-throughput assay

Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions.

• 出版日期2015-5-3