A statistical analysis on transcriptome sequences: The enrichment of Alu-element is associated with subcellular location

作者：Chen, Ke^*; Wang, Yuxuan; Sun, Jie

来源：Biochemical and Biophysical Research Communications, 2018, 499(3): 397-402.

DOI：10.1016/j.bbrc.2018.03.024

• Alu-element
• Subcellular location
• Transcriptome
• Statistical analysis

The Alu-element plays important roles in mediating alternative splicing, RNA editing and translation regulation. However, the distribution and function of the Alu-element are never analysed at the transcriptome level. This study presents a statistical analysis of the Alu-element on human transcriptome. We found that mRNAs and lncRNAs share the same sequence form for the Alu-element. The Alu-element covers 5.8% of the coding transcripts and 17.1% of the coding genes for mRNAs, and covers 9.3% of the transcripts and 13.6% of the genes for IncRNAs. The Alu-element is preferentially located at the 3' end. Statistical analysis demonstrates that the enrichment of Alu-element is associated with subcellular location. For instance, Alu-inclusive transcripts are overexpressed in nucleus, mitochondrion and Golgi apparatus membrane while under-expressed in cell membrane and extracellular space. We found that genes contain both Alu-element and S-domains of 7SL RNA are all associated with cellular activities carried out in mitochondrion.

• 出版日期2018-5-15
• 单位天津工业大学