科研之友
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摘要
A Gram-negative, aerobic, motile, rod-shaped, pale-yellow bacterial strain, designated as ZYL(T), isolated from a cultured in situ sediment sample collected from the East China Sea coast, was studied using a polyphasic taxonomic approach. Strain ZYL(T) grew at 4-30 degrees C (optimum, 25 degrees C), at pH 6.0-8.5 (pH 7.0) and with 0-7.0% (w/v) NaCl (2.0 %). Results of phylogenetic analysis based on 16S rRNA gene sequences clearly showed that strain ZYL(T) and Emcibacter nanhaiensis HTCJW17(T), which was most closely related to strain ZYL(T) with 93.6% sequence similarity, clustered together. The genomic DNA G+C content was 51.5% (genome sequence). The quinone system was composed only of ubiquinone-10. Strain ZYL(T) possessed C-18 : 1 omega 7c and/or C-18 : 1 omega 6c (summed feature 8), iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c (summed feature 3), C-14 : 0 2-OH and C-14 : 0 as the major fatty acids. The content of summed feature 3 (iso-C-15 : 0 2-OH and/or C-16 : 1 omega 7c) in strain ZYL(T) was far greater than that in E. nanhaiensis. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, three unidentified phospholipids, one unidentified aminolipid and four unidentified lipids. One unidentified aminophospholipid and two unidentified lipids present in strain ZYL(T) were not found in E. nanhaiensis in this research. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain ZYL(T) (=KCTC 62328(T) =JCM 32378(T) =MCCC 1K03526(T)) represents a novel species of the genus Emcibacter for which the name Emcibacter congregatus sp. nov. is proposed.
