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摘要
This report shows that pig manure can be used for algal production in small farms. Huge pig sludge amounts produced using actual methods in large-scale pig breeding farms is a major issue due to the lack of disposal options and potential water pollution. This issue may be solved by using pig sludge for algal biofuel production. Therefore, we studied an economical method of algae production on pig sludge that can be operated on animal farms in Hungary with modest levels of investment. We analyzed four algae species, Chlorella vulgaris, Scenedesmus quadricauda, Scenedesmus dimorphus, and Arthrospira platensis, in the laboratory and C. vulgaris in outdoor conditions. The following parameters were studied: pot size, illumination, temperature, filtered versus unfiltered pig manure, water depth, aeration, CO2 enrichment, inoculums solution, fertilization level, and length of rotation period. Produced alga biomass was measured every 4 days, and the protein- and lipid content was analyzed using the method of Bradford (1976) and Erickson (1993). Our results show first that the unfiltered pig manure is not suitable for algae production due to illumination shortage. By contrast, using filtered pig manure, we found that C. vulgaris yield in 70-1 indoor pots was 64% higher than in smaller pots. In a larger outdoor system, the annual dry yield of C. vulgaris reached 141-259 t/ha during 12-day-long rotation periods. Here, we demonstrate that only filtered liquid pig manure can be recommended for algae production. C. vulgaris is the most suitable alga species for use in large-scale experiments on pig sludge. Based on our outdoor experiments, the adaptation of a 12- to 14-day rotation period could be considered the most reasonable. An algae farm can therefore be operated with relative modest amount of capital, can hence address the issue of sludge management, and provide a substrate for energy production.
- 出版日期2012-7
