High transformation efficiency of Bacillus subtilis with integrative DNA using glycine betaine as osmoprotectant

作者：Meddeb Mouelhi Fatma; Dulcey Carlos; Beauregard Marc^*

来源：Analytical Biochemistry, 2012, 424(2): 127-129.

DOI：10.1016/j.ab.2012.01.032

• Bacillus subtilis
• Integrative plasmid
• Electroporation
• Transformation efficiency
• Glycine betaine
• Osmolarity
• Electrocompetent cells

Electroporation is an important approach for genetic engineering experiments allowing for introduction of foreign DNA in a selected host. Here, we describe for the first time the use of glycine betaine as an osmoprotectant for electroporation of gram-positive bacteria Bacillus subtilis. High electroporation efficiency (up to 5 x 10(5) cfu/mu g) was obtained using 7.5% glycine betaine. The new method improved the transformation efficiency of B. subtilis with linear integrative DNA nearly 700-fold compared with existing Bacillus transformation techniques.

• 出版日期2012-5-15