科研之友
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摘要
The Candida albicans cell wall provides an architecture that allows for the organism to survive environmental stress as well as interaction with host tissues. Previous work has focused on growing C. albicans on media such as Sabouraud or YPD at 30 degrees C. Because C albicans normally colonizes a host, we hypothesized that cultivation on blood or serum at 37 degrees C would result in structural changes in cell wall mannan. C. albicans SC5314 was inoculated onto YPD, 5% blood, or 5% serum agar media three successive times at 30 degrees C and 37 degrees C, then cultivated overnight at 30 degrees C in YPD. The mannan was extracted and characterized using 1D and 2D 1H NMR techniques. At 30 degrees C cells grown in blood and serum contain less acid-stable terminal beta-(1 -> 2)-linked D-mannose and alpha-(1 -> 2)-linked D-mannose-containing side chains, while the acid-labile side chains of mannan grown in blood and serum contain fewer beta-Man-(1 -> 2)-alpha-Man-(1 -> side chains. The decrement in acid-stable mannan side chains is greater at 37 degrees C than at 30 degrees C. Cells grown on blood at 37 degrees C show fewer -> 6)-alpha-Man-(1 -> structural motifs in the acid-stable polymer backbone. The data indicate that C albi cans, grown on media containing host-derived components, produces less complex mannan. This is accentuated when the cells are cultured at 37 degrees C. This study demonstrates that the C albicarts cell wall is a dynamic and adaptive organelle, which alters its structural phenotype in response to growth in host-derived media at physiological temperature.
- 出版日期2011-12-13
