A new pyrene-based fluorescent probe with large Stokes shift for detecting hydrogen peroxide in aqueous solution and living cells

作者：Nie, Jing; Liu, Yong; Niu, Jie; Ni, Zhonghai^*; Lin, Weiying^*

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2017, 348: 1-7.

DOI：10.1016/j.jphotochem.2017.08.008

• Fluorescent probe
• Stokes shift
• Hydrogen peroxide

Hydrogen peroxide (H2O2) plays an important role in signal transduction and therapy of serious diseases. In this work, we have developed a new pyrene-based fluorescent probe Py-Boe, which possesses large Stokes shift and achieves detection of H2O2 in aqueous solution. The probe Py-Boe exhibits excellent photostability, high sensitive and selectivity. Taking advantage of these properties, the probe Py-Boe achieves succussfully imaging of exogenous and endogenous H2O2 in living RAW 264.7 cells. We expect that this dye with large Stokes shift may open an avenue to construct novel fluorescent probe with large Stokes shift for biological application.

• 出版日期2017-11-1
• 单位中国矿业大学（北京）; 济南大学