A RAPD-PCR Method for the Rapid Detection of Bacillus cereus

作者:Lee Jiyeon; Kwon Gun Hee; Park Jae Yong; Park Cheon Seok; Kwon Dae Young; Lim Jinkyu; Kim Jong Sang; Kim Jeong Hwan*
来源:Journal of Microbiology and Biotechnology, 2011, 21(3): 274-276.
DOI:10.4014/jmb.1008.08031

摘要

Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.

  • 出版日期2011-3