Studies on genetic variability in Alternaria alternata (Fr.) Keissler causing leaf blight of Isabgol by RAPD

作者：Kumar Meena Rajeah^*; Sharma S S

来源：Research Journal of Biotechnology, 2014, 9(8): 38-44.

• Genetic variability
• Alternaria alternata
• Plantago ovata and RAPD

Five Alternaria alternata isolates from Isabgol (Plantago ovata) plants of different places i.e. Udaipur, Chittorgarh, Jodhpur, Pali and Jalore district of Rajasthan were analyzed for genetic diversity by using Random Amplified Polymorphic DNA (RAPD). A total of 94 amplified bands were obtained and 68 were polymorphic. The dendrogram based on RAPD analysis has generated only one group. However two isolates i.e. Aa-1 and Aa-4 which belonged to RCA (Udaipur) and Sumerpur (Pali) did not cluster with the group.
The group consisted of three isolates i.e. Aa-2, Aa-3 and Aa-5 and had higher group similarity of 68.00 per cent. Two isolates viz. Aa-1 and Aa-4 joined this group at a similarity of 54.00 per cent and 55.00 per cent respectively. The primers OPA-9, OPA-3 and OPA-17 gave 91.66%, 87.50% and 87.50% polymorphism of the DNA respectively which helped to ascertain variability. Therefore, these primers could be very useful for ascertaining variability among the population of Alternaria alternata.

• 出版日期2014-8