A new strategy for the passive skin delivery of nanoparticulate, high molecular weight hyaluronic acid prepared by a polyion complex method

作者：Tokudome Yoshihiro; Komi Tatsuya; Omata Ami; Sekita Marie

来源：Scientific Reports, 2018, 8(1): 2336.

DOI：10.1038/s41598-018-20805-3

<jats:title>Abstract</jats:title><jats:p>Restoring hyaluronic acid (HA) content is important for maintaining the function of photo-aged skin. This study aimed to evaluate the passive delivery into skin of HA nanoparticles formed by the polyion complex method. Nanoparticles were prepared by mixing and stirring anionic HA with a cationic polymer, protamine, at the charge ratio 55:45. The permeation of fluorescently-labelled HA nanoparticles (HANP) or free HA through hairless mouse skin was characterized <jats:italic>in vitro</jats:italic>. HANP or free HA was applied to ultraviolet (UV)-irradiated mice <jats:italic>in vivo</jats:italic>, and their transepidermal water loss (TEWL) was measured after 4 days. HA that had been delivered into skin was separated and characterized by molecular sieve chromatography. HANP were able to deliver HA into the dermis both <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>, whereas free HA penetrated no further than the stratum corneum. Following HANP application, HA within the skin was present in the form of free HA rather than nanoparticles. When applied <jats:italic>in vivo</jats:italic>, HANP significantly reduced the TEWL caused by UV irradiation. Thus, although free HA does not penetrate into the skin by passive diffusion, HA can be effectively delivered by nanoparticles. HA is then released from the nanoparticles and can contribute to barrier recovery following UV irradiation.</jats:p>

• 出版日期2018-2-5