Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring

作者：Robinson Robert; Wong Liam; Monnat Raymond J Jr; Fu Elain^*

来源：Micromachines, 2016, 7(2): 28.

DOI：10.3390/mi7020028

• paper-based device
• whole-blood assay
• phenylalanine detection
• colorimetric readout
• phenylketonuria

Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 L) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients.

• 出版日期2016-2