DEFB1 polymorphisms and salivary hBD-1 concentration in Oral Lichen Planus patients and healthy subjects

作者：Polesello Vania; Zupin Luisa; Di Lenarda Roberto; Biasotto Matteo; Pozzato Gabriele; Ottaviani Giulia; Gobbo Margherita; Crovella Sergio; Segat Ludovica^*

来源：Archives of Oral Biology, 2017, 73: 161-165.

DOI：10.1016/j.archoralbio.2016.10.008

• Human beta defensin 1
• Saliva
• DEFB1
• Polymorphisms
• ELISA
• Oral Lichen Planus

Objectives: The aetiology of Oral Lichen Planus (OLP), a chronic inflammatory disease of oral mucosa, is not yet well understood. Since innate immunity may be hypothesized as involved in the susceptibility to OLP, we studied human beta defensin 1 (hBD-1) an antimicrobial peptide constitutively expressed in the saliva, looking at functional genetic variants possibly able to diminish hBD-1 production an consequently conferring major susceptibility to OLP.
Design: We analysed three DEFB1 polymorphisms at 5' UTR, -52G > A (rs1799946), -44C > G (rs1800972), -20G > A (rs11362) and two DEFB1 polymorphisms at 3'UTR, c*5G > A (rs1047031), c*87A > G (rs1800971), with the aim of correlating these genetic variants and hBD-1 salivary level in a group of OLP patients and in healthy subjects. We also evaluated hBD-1 salivary concentrations, using ELISA, in OLP and healthy controls.
Results: We compared hBD-1 concentrations in OLP and healthy subjects: hBD-1 concentration was significantly higher in OLP patients respect to control. When considering the correlation between DEFB1 polymorphisms genotypes and hBD-1 expression levels, significant results were obtained for SNPs -52G > A (p = 0.03 both in OLP patients and healthy individuals) and -44C > G (p = 0.02 in OLP patients).
Conclusions: hBD-1 production was different between OLP and healthy subjects (not age-matched with OLP). DEFB1 gene polymorphisms, -52G > A and -44C > G, correlated with hBD-1 salivary concentrations.

• 出版日期2017-1