A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

作者：Runyon J Ray; Ulmius Matilda; Nilsson Lars^*

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2014, 442: 25-33.

DOI：10.1016/j.colsurfa.2013.04.010

• Field-flow fractionation
• Colloids
• Proteins
• Beta-glucans
• Aggregates
• Emulsions

Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (similar to 2 nm to 50 mu m in diameter) and molecular weight range (10(3)-10(10) g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets.

• 出版日期2014-2-1