Assessment of repeatability of composition of perfumed waters by high-performance liquid chromatography combined with numerical data analysis based on cluster analysis (HPLC UV/VIS-CA)

作者：Ruzik L^*; Obarski N; Papierz A; Mojski M

来源：International Journal of Cosmetic Science, 2015, 37(3): 348-356.

DOI：10.1111/ics.12195

• cluster analysis
• HPLC
• perfumed waters
• quality control
• repeatability of composition

Synopsis High-performance liquid chromatography (HPLC) with UV/VIS spectrophotometric detection combined with the chemometric method of cluster analysis (CA) was used for the assessment of repeatability of composition of nine types of perfumed waters. In addition, the chromatographic method of separating components of the perfume waters under analysis was subjected to an optimization procedure. The chromatograms thus obtained were used as sources of data for the chemometric method of cluster analysis (CA). The result was a classification of a set comprising 39 perfumed water samples with a similar composition at a specified level of probability (level of agglomeration). A comparison of the classification with the manufacturer's declarations reveals a good degree of consistency and demonstrates similarity between samples in different classes. A combination of the chromatographic method with cluster analysis (HPLC UV/VIS - CA) makes it possible to quickly assess the repeatability of composition of perfumed waters at selected levels of probability.
Resume La chromatographie liquide a haute performance (CLHP) avec detection UV / VIS spectrophotometrique combinee avec la methode chimiometrique d'analyse de cluster (CA) a ete utilise pour l'evaluation de la repetabilite de la composition de neuf types d'eaux parfumees. En outre, le procede de separation chromatographique des composants des eaux parfumees analysees a ete soumis a une procedure d'optimisation. Les chromatogrammes ainsi obtenus ont ete utilises comme sources de donnees pour la methode chimiometrique d'analyse de cluster (CA). Le resultat a ete une classification d'un ensemble comprenant 39 echantillons d'eau parfumees avec une composition similaire a un niveau de probabilite specifique (niveau de l'agglomeration). Une comparaison de la classification avec les declarations du fabricant revele un bon degre de coherence et demontre la similitude entre les echantillons dans differentes classes. Une combinaison de la methode chromatographique avec l'analyse de cluster (HPLC UV / VIS - CA) permet d'evaluer rapidement la repetabilite de la composition des eaux parfumees a des niveaux de probabilite selectionnes.

• 出版日期2015-6