The Nop5-L7A-fibrillarin RNP complex and a novel box C/D containing sRNA of Halobacterium salinarum NRC-1

作者：Weisel Jasmin; Wagner Steffen^*; Klug Gabriele

来源：Biochemical and Biophysical Research Communications, 2010, 394(3): 542-547.

DOI：10.1016/j.bbrc.2010.03.012

• Box C/D
• Archaea
• Halobacterium
• Nop5
• L7A
• Fibrillarin

RNA 2'O-methylation is a frequent modification of rRNA and tRNA and supposed to influence RNA folding and stability Ribonucleoprotein (RNP) complexes, containing the proteins Nop5, L7A, fibrillarin, and a box C/D sRNA. are guided for 2'O-methylation by interactions of their RNA component with their target RNA In vitro complex assembly was analyzed for several thermophilic Archaea but in vivo studies are rare, even unavailable for halophilic Archaea To analyze the putative box C/D RNP complex in the extremely halophilic Halobacterium salinarum NRC-1 we performed pull-clown analysis and identified the proteins Nop5, L7A, and fibrillarin and the tRNA(Trp) intron, as a typical box C/D sRNA of this RNP complex in vivo. We show for the first time a ribonucleolytic activity of the purified RNP complex proteins, as well as for the RNP complex containing pull-down fractions. Furthermore, we identified a nov

• 出版日期2010-4-9