Development of New Fluorescent Dyes by Using Amine Modified Nanoparticles for Immunostaining

作者：Maltas Esra^*; Ozmen Mustafa; Ucan H Ismet

来源：Synthesis and Reactivity in Inorganic Metal-Organic and Nano-Metal Chemistry, 2016, 46(12): 1779-1786.

DOI：10.1080/15533174.2015.1137062

• calf-thymus DNA
• fluorescence
• ligand
• magnetic nanoparticle

Fluorescent labeling of DNA was studied by using two newly synthesized ligands, which are 4-(2-hydroxybenzylidinamino) benzoic acid (PHBA) and 4-(2-hydroxybenzylidinamino) phthalic acid (PHPA). Their binding capacity to calf-thymus DNA was determined on functionalized superparamagnetic iron oxide nanoparticles (SPIONs). 23.1 mu g of DNA was immobilized on 25mg of nanoparticles. Binding capacity of fluorescent ligands to DNA-APTS-SPIONs was also found as 2.36 mu M for PHBA and 1.97 for PHPA at 25mg of the nanoparticles. Binding of the DNA to the ligands were examined by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, and confocal microscopy.

• 出版日期2016