A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets

作者：Khorshid Mohsen; Hausser Jean; Zavolan Mihaela^*; van Nimwegen Erik

来源：Nature Methods, 2013, 10(3): 253-255.

DOI：10.1038/NMETH.2341

We introduce a biophysical model of miRNA-target interaction and infer its parameters from Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction of human miRNA target sites are noncanonical and that predicted target-site affinity correlates well with the extent of target destabilization. Our model provides a rigorous biophysical approach to miRNA target identification beyond ad hoc miRNA seed based methods.

• 出版日期2013-3