Annexin A4 is a possible biomarker for cisplatin susceptibility of malignant mesothelioma cells

作者：Yamashita Takuya; Nagano Kazuya; Kanasaki So ichiro; Maeda Yuka; Furuya Takeshi; Inoue Masaki; Nabeshi Hiromi; Yoshikawa Tomoaki; Yoshioka Yasuo; Itoh Norio; Abe Yasuhiro; Kamada Haruhiko; Tsutsumi Yasuo; Tsunoda Shin ichi^*

来源：Biochemical and Biophysical Research Communications, 2012, 421(1): 140-144.

DOI：10.1016/j.bbrc.2012.03.144

• Malignant mesothelioma
• Cisplatin susceptibility
• Annexin A4
• Biomarker
• Proteomics

Mesothelioma is a highly malignant tumor with a poor prognosis and limited treatment options. Although cisplatin (CDDP) is an effective anticancer drug, its response rate is only 20%. Therefore, discovery of biomarkers is desirable to distinguish the CDDP-susceptible versus resistant cases. To this end, differential proteome analysis was performed to distinguish between mesothelioma cells of different CDDP susceptibilities, and this revealed that expression of annexin A4 (ANXA4) protein was higher in CDDP-resistant cells than in CDDP-susceptible cells. Furthermore, ANXA4 expression levels were higher in human clinical malignant mesothelioma tissues than in benign mesothelioma and normal mesothelial tissues. Finally, increased susceptibility was observed following gene knockdown of ANXA4 in mesothelioma cells, whereas the opposite effect was observed following transfection of an ANXA4 plasmid. These results suggest that ANXA4 has a regulatory function related to the cisplatin susceptibility of mesothelioma cells and that it could be a biomarker for CDDP susceptibility in pathological diagnoses.

• 出版日期2012-4-27