Alterations of Global Histone H3K9 and H3K27 Methylation Levels in Bladder Cancer

作者：Ellinger Joerg^*; Bachmann Anne; Goeke Friederike; Behbahani Turang E; Baumann Claudia; Heukamp Lukas C; Rogenhofer Sebastian; Mueller Stefan C

来源：Urologia Internationalis, 2014, 93(1): 113-118.

DOI：10.1159/000355467

• Histone
• Methylation
• Bladder cancer
• H3K9
• H3K27

Background: Epigenetic alterations, including histone modifications, play an important role during carcinogenesis. This study was designed to systematically investigate histone H3K9 and H3K27 methylation levels in bladder cancer (BCa) tissue. Methods:A tissue microarray with urothelial BCa (150 non-muscle-invasive BCa, NMIBC; 121 muscle-invasive BCa, MIBC; 31 metastatic BCa, MET) and normal urothelium (29, CTRL) specimen was used to determine the global levels of H3K9 and H3K27 mono-, di- and tri-methylation. Results: Global levels of H3K9 and H3K27 methylation were significantly higher in CTRL than in BCa, and levels in NMIBC were higher compared to MIBC. Histone nnethylation levels of MET resembled MIBC. We observed furthermore a correlation of histone nnethylation levels with pT stage (H3K9me1, H3K9me2, H3K9me3, H3K27me1, H3K27me3) and grade (H3K9nne2, H3K9me3, H3K27nne1) in NMIBC. H3K9me1, H3K9me3, H3K27me1 and H3K27me3 levels were also correlated with pT stage in MIBC. Histone modifications were not associated with recurrence-free or cancer-specific survival. Conclusions: Global histone H3K9 and H3K27 nnethylation levels are altered in BCa.

• 出版日期2014