Anti cervix cancer activity of co-immobilized tumor necrosis factor-alpha and interferon-gamma

作者：Guan, YQ^*; He, LM; Cai, SM; Zhou, TH

来源：Journal of Materials Science & Technology, 2006, 22(2): 200-204.

DOI：10.3321/j.issn:1005-0302.2006.02.012

• tumor necrosis factor (TNF-alpha)
• interferon-gamma (IFN-gamma)
• cervix cancer cell line
• photo-immobilization
• polystyrene
• inhibitory activity

Tumor necrosis factor alpha (TNF-alpha) and interferon-gamma (IFN-gamma) are cytokines with strong antitumor activities. They were reacted with a photoactive arylazide-4-azidobenzoic acid, resulting in photoactive TNF-alpha and IFN-gamma. The infrared (IR) spectra of these products showed the characteristic absorption of an azido group at 2127 cm(-1). By photo-immobilization, this modified TNF-alpha and IFN-gamma were immobilized on polystyrene membranes for cell culture to prepare biomaterials. The micro-morphology of photoactive cytokines was observed with a scanning electron microscope (SEM). The inhibitory effect on growth of Hela cells and inducing apoptosis activity of these two cytokines were analyzed by growth curve, transmission electron microscope (TEM) and fluorescence active cell sorter (FACS). The results showed that co-immobilization of IFN-gamma and TNF-alpha had significant inhibitory effect on growth of Hela cells, inhibitory rate up to 82%, and IFN-gamma had obviously synergistic action.

• 出版日期2006-3
• 单位暨南大学; 华南师范大学