Disposable luminol copolymer-based biosensor for uric acid in urine

作者：Ballesta Claver J; Diaz Ortega I F; Valencia Miron M C; Capitan Vallvey L F^*

来源：Analytica Chimica Acta, 2011, 702(2): 254-261.

DOI：10.1016/j.aca.2011.06.054

• Electrochemiluminescence
• Disposable biosensor
• Conducting polymers
• Luminol copolymer
• Uric acid determination
• Urine samples

A new electrochemiluminescent (ECL) disposable biosensor for uric acid was manufactured by immobilization in a double-layer design of luminol as a copolymer with 3,3',5,5'-tetramethylbenzidine (TMB) and the enzyme uricase in chitosan on gold screen-printed cells. The good mechanical and improved electroluminescent characteristics of the new copolymer poly(luminol-TMB) make it possible to determine uric acid by measuring the growing ECL emission with the analyte concentration. The combination of enzymatic selectivity with ECL sensitivity results in a disposable analytical device with a linear range for uric acid from 1.5 x 10(-6) to 1.0 X 10(-4) M, a limit of detection of 4.4 X 10(-7) M and a precision of 13.1% (1.0 x 10(-5) M, n = 10) as relative standard deviation. Satisfactory results were obtained for uric acid determination in 24 h-urine samples compared to a reference procedure. This uric acid biosensor can be used as a low-cost alternative to conventional methods.

• 出版日期2011-9-30