An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

作者：Zhao Qiang^*; Gao Jie

来源：Chemical Communications, 2013, 49(70): 7720-7722.

DOI：10.1039/c3cc44400g

Here we present a sensitive and specific assay for thrombin through the affinity capture of thrombin with mRNA display generated peptide aptamers on magnetic beads and the subsequent thrombin-involved enzymatic reaction. Thrombin at 50 fM can be detected.

• 出版日期2013
• 单位山西大学