A U-Tetrad Stabilizes Human Telomeric RNA G-Quadruplex Structure

作者：Xu Yan^*; Ishizuka Takumi; Kimura Takashi; Komiyama Makoto

来源：Journal of the American Chemical Society, 2010, 132(21): 7231-+.

DOI：10.1021/ja909708a

Telomeric repeat-containing RNA is a new noncoding RNA molecule recently discovered in mammalian cells. Here we report the structural features of human telomere RNA r(UAGGGU) in the presence of K(+) and Na(+). We demonstrated for the first time that a novel U-tetrad is formed at the 3' end of a parallel human telomeric RNA G-quadruplex. The U-tetrad dramatically stabilizes human telomeric RNA G-quadruplex structure, leading to an increase in melting temperature (T(m)) of 29 degrees C. The U-tetrad-stabilized telomeric RNA G-quadruplex structure adds considerably to our understanding of the diversity of RNA G-quadruplex architectures. It shows that the structure of base "quartets" is important in RNA assembly. The structural information will be invaluable for understanding the function of human telomere RNA.

• 出版日期2010-6-2