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摘要
An effective thin-layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of thiamphenicol (TAP) in pig, chicken, and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C-18 column using an isocratic procedure with acetonitrile-water (21.7:78.3, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The TAP concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=162,630x-2381.7, r>0.9998) were achieved within the concentration range of 0.05-10.00g/mL. The recoveries of TAP spiked at levels of 1, 10, and 100g/g ranged from 82.0 to 114.9% with the intra-day and inter-day relative standard deviation (RSD) less than 9.0%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.03mg/kg for pig feedstuffs, 0.02 and 0.04mg/kg for chicken feedstuffs, and 0.02 and 0.03mg/kg for fish feedstuffs, respectively. This reliable, simple, and cost-effective method could be applied to the routine monitoring of TAP in animal feedstuffs.
- 出版日期2018-11
- 单位湖北省农业科学院; 浙江省农业科学院
