Absorption and Bioavailability of Nano-Size Reduced Calcium Citrate Fortified Milk Powder in Ovariectomized and Ovariectomized-Osteoporosis Rats

作者：Erfanian Arezoo; Mirhosseini Hamed; Rasti Babak; Hair Bejo Mohd; Bin Mustafa Shuhaimi; Abd Manap Mohd Yazid^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(24): 5795-5804.

DOI：10.1021/acs.jafc.5b01468

• calcium bioavailability
• calcium absorption
• nanoparticle
• osteoporosis
• in vivo study
• menopause
• fortified milk

The aim of this study was to evaluate the effects of fortification and nano-size reduction on calcium absorption and bioavailability of milk powder formula in sham, ovariectomized, and ovariectomized-osteoporosis rats as a menopause and menopause-osteoporosis model. Skim milk powder and skim milk powder fortified with calcium citrate and the suitable doses of inulin, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and vitamins D-3, K-I and B-6 were formulated based on the North American and Western European recommended dietary allowances. Optimization on cycle and pressure of high-pressure homogenizer was done to produce nano-fortified milk powder. In vivo study demonstrated that fortification and calcium citrate nano-fortified milk powder increased absorption and bioavailability of calcium, as well as bone stiffness and bone strength in sham, ovariectomized, and ovariectomized-osteoporosis rats. This study successfully developed an effective fortified milk powder for food application.

• 出版日期2015-6-24