microRNA-34a-Upregulated Retinoic Acid-Inducible Gene-I Promotes Apoptosis and Delays Cell Cycle Transition in Cervical Cancer Cells

作者：Wang, Jing-Hua; Zhang, Le; Ma, Yu-Wei; Xiao, Jing; Zhang, Yi; Liu, Min; Tang, Hua^*

来源：DNA and Cell Biology, 2016, 35(6): 267-279.

DOI：10.1089/dna.2015.3130

• HEPATOCELLULAR-CARCINOMA CELLS
• DOUBLE-STRANDED-RNA
• RIG-I
• ANTIVIRAL RESPONSES
• IMMUNE-RESPONSE
• DOWN-REGULATION
• FEEDBACK LOOP
• VIRAL-RNA
• INVASION
• PROLIFERATION

The function of retinoic acid-inducible gene-I (RIG-I) in viral replication is well documented, but its function in carcinogenesis and malignancies as well as relationship with microRNAs (miRNAs) remain poorly understood. miR-34a is an antioncogene in multiple tumors. In our study, RIG-I and miR-34a suppressed cell growth, proliferation, migration, and invasion in cervical cancer cells in vitro. miR-34a was validated as a new regulator of RIG-I by binding to its 3' untranslated region and upregulating its expression level. Furthermore, we revealed that RIG-I and miR-34a enhanced apoptosis, delayed the G1/S/G2 transition of the cell cycle, and inhibited the epithelial-mesenchymal transition process to modulate malignancies in cervical cancer cells. Phenotypic rescue experiments indicated that RIG-I mediates the effects of miR-34a in HeLa and C33A cells. These findings provide new insights into the mechanisms that underlie carcinogenesis and may provide new biomarkers for the diagnosis and therapy of cervical cancer.

• 出版日期2016-6
• 单位天津医科大学