A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy

作者：Hoang Le D; Smeenk L; Anraku I; Pijlman G P; Wang X J; de Vrij J; Liu W J; Le T T; Schroder W A; Khromykh A A; Suhrbier A^*

来源：Gene Therapy, 2009, 16(2): 190-199.

DOI：10.1038/gt.2008.169

• Kunjin replicon
• granulocyte macrophage colony-stimulating factor
• tumour

We have recently developed a non-cytopathic RNA replicon-based viral vector system based on the flavivirus Kunjin. Here, we illustrate the utility of the Kunjin replicon system for gene therapy. Intra-tumoral injections of Kunjin replicon virus-like particles encoding granulocyte colony-stimulating factor were able to cure >50% of established subcutaneous CT26 colon carcinoma and B16-OVA melanomas. Regression of CT26 tumours correlated with the induction of anticancer CD8 T cells, and treatment of subcutaneous CT26 tumours also resulted in the regression of CT26 lung metastases. Only a few immune-based strategies are able to cure these aggressive tumours once they are of a reasonable size, illustrating the potential of this vector system for intra-tumoral gene therapy applications.

• 出版日期2009-2
• 单位河北医科大学