科研之友
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摘要
Essential oils of Litsea cubeba, Litsea salicifolia and Melaleuca cajuputi were tested against Luciaphorus perniciosus by contact and fumigation methods. Contact toxicity bioassay was made by dropping essential oils at rates of 0, 0.06, 0.33, 0.66, 3.3, 6.6, 33, 66 and 99 mu l/cm(2) into special mite cages. Fumigation was done in a knockdown chamber of 2.5x10(4) cm(3) in size at the concentrations of 0, 0.0012, 0.006, 0.012, 0.06, 0.12, 0.6 and 1.2 mu g/cm(3). The essential oil of L. cubeba was found to be the most toxic to L. perniciosus by both contact and fumigation methods with LD(50) values equaling to 0.932 and 0.166 mu g/cm(3), respectively, followed by essential oil of L. salicifolia whose LD(50) values of 2.793 and 0.410 mu g/cm(3) were recorded. Essential oil of M. cajuputi demonstrated a low effect on L. perniciosus as compared to those of L. cubeba and L. salicifolia. Essential oil formulations were delivered by using citronella grass or black pepper oils as major components and L. cubeba as minor component. Mite mortality rate increased when 0.01% L. cubeba oil was added to either citronella grass or black pepper oil at all treatments by contact method.
- 出版日期2010-6
