Arginine vasopressin remolds the spontaneous discharges disturbed by amyloid beta protein in hippocampal CA1 region of rats

作者：Pan Yan Fang; Jia Xiao Tao; Wang Xiao Hui; Chen Xiao Rong; Li Qing Shan; Gao Xiu Ping; Qi Jin Shun^*

来源：Regulatory Peptides, 2013, 183: 7-12.

DOI：10.1016/j.regpep.2013.03.003

• Arginine vasopressin (AVP)
• Amyloid beta-protein (A beta)
• Multi-channel extracellular recording
• Spontaneous discharge
• Hippocampus
• In vivo

Beta-amyloid peptide (A beta) aggregated in the brain is the main pathological characteristic of Alzheimer's disease (AD), and a significant decrease in the concentration of arginine vasopressin (AVP) in the brain of AD patients has been reported. Our recent study shows that intracerebroventricular (i.c.v.) injection of AVP protects against A beta-induced impairments of spatial learning and memory. However, it is still unclear whether the A beta-induced cognitive deficit is involved in the alteration of central neuronal discharges, and further whether AVP can modulate the electrophysiological change induced by A beta. The present study thus observed the effects of AVP, A beta and AVP plus A beta on the spontaneous discharges of hippocampal CA1 neurons in rats by using multi-channel extracellular recording technique. The results showed that: (1) the average frequency of spontaneous discharges was decreased by i.c.v. injection of 25 nmol A beta(25-35); (2) 10 nmol AVP induced an increase in spike discharge in the hippocampal CA1 neurons: (3) pretreatment with 10 nmol AVP effectively reversed A beta(25-35) induced suppression of spontaneous discharges in hippocampal CA1 region. These in vivo electrophysiological results indicate that AVP, as a hormone and neurotransmitter, can remold the spontaneous discharges disturbed by A beta and counteract the deleterious effect of A beta(25-35) on neural circuit, suggesting that the activation of central vasopressinergic system may play a beneficial role for the prevention and treatment of cognitive impairments in AD.

• 出版日期2013-5-10
• 单位北京协和医学院; 山西医科大学; 中国医学科学院北京协和医院