A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes

作者：Kang, Xinhuang; Mai, Zhibin; Zou, Xiaoyong^*; Cai, Peixiang; Mo, Jinyuan

来源：Analytical Biochemistry, 2007, 369(1): 71-79.

DOI：10.1016/j.ab.2007.07.005

• glucose
• carbon nanotubes
• Au-Pt alloy nanoparticles
• chitosan
• electrodeposition
• biosensor

A novel glucose biosensor was constructed, based on the immobilization of glucose oxidase (GOx) with cross-linking in the matrix of biopolymer chitosan (CS) on a glassy carbon electrode (GCE), which was modified with gold-platinum alloy nanoparticles (Au-PtNPs) by electrodeposition on multiwall carbon nanotubes (CNTs) in CS film (CNTs/CS). The properties of Au-PtNPs/CNTs/CS were characterized by scan electron microscopy (SEM), X-ray diffraction (XRD), cyclic voltarnmetry (CV), and electrochemical impedance spectra (EIS). Primary study indicated that Au-PtNPs/CNTs had a better synergistic electrocatalytic effect on the reduction of hydrogen peroxide than did AuNPs/CNTs or PtNPs/CNTs at a low applied potential With GOx as a model enzyme, a new glucose biosensor was fabricated. The biosensor exhibited excellent performances for glucose at a low applied potential (0.1 V) with a high sensitivity (8.53 mu A mM(-1)), a low detection limit (0.2 mu M), a wide linear range (0.001-7.0 mM), a fast response time (< 5 s), and good reproducibility, stability, and selectivity. In addition, the biosensor was applied in the determination of glucose in human blood and urine samples, and satisfied results were obtained.

• 出版日期2007-10-1
• 单位广东海洋大学; 中山大学