Layer-by-layer assembled protein nanotubes with high DNA affinity

作者：Jiao, Peipei; Guo, Yanli^*; Niu, Aihua; Kang, Xiaofeng

来源：RSC Advances, 2015, 5(47): 37130-37137.

DOI：10.1039/c5ra04725k

• GENE DELIVERY
• CARBON NANOTUBES
• ADSORPTION
• IMMOBILIZATION
• NANOPARTICLES
• BINDING
• FIELD
• DYE

In this research, protein nanotubes with high affinity for DNA molecules were prepared by alternate layer-bylayer ( LbL) assembly of human serum albumin (HSA) and polyethylenimine (PEI). The well-defined hollow cylinder (PEI/HSA) 5PEI nanotubes with outer diameter 455 +/- 13 nm, inner diameter 275 +/- 35 nm, wall thickness 151 +/- 7 nm and length 5.6 +/- 0.9 mm were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDS). Under optimal conditions, the maximum adsorption capacity of protein nanotubes for polyA25 was 606.0 mg g(-1), which was far higher than the reported nanoparticles. The rates of adsorption were found to conform to the pseudo-second-order kinetics and intra-particle diffusion models with good correlation. The equilibrium adsorption data fitted well with the Langmuir isotherm model. The DNA release fromDNA/(PEI/HSA) 5PEI nanotubes was associatedwith the ionic strength and pHin the solution.

• 出版日期2015
• 单位西北大学