Antiviral activity of a polysaccharide from Laminaria japonica against enterovirus 71

作者：Yue, Yingying; Li, Zhihui; Li, Peng; Song, Nannan; Li, Bingqing; Lin, Wei; Liu, Shuntao^*

来源：Biomedicine & Pharmacotherapy, 2017, 96: 256-262.

DOI：10.1016/j.biopha.2017.09.117

• Enterovirus 71
• Antiviral activity
• Polysaccharide
• Apoptosis
• IFN-beta

This in vitro study investigated the antiviral activity of an acidic polysaccharide from Laminaria japonica against enterovirus 71 ( EV71) as well as its mechanism of action. The LJ04 polysaccharide was purified from Laminaria japonica by affinity chromatography. To investigate its antiviral activity, an MTT assay, q-PCR, immunofluorescent staining and western-blot analysis were performed. To define its mechanism of action, ELISA, q-PCR and flow cytometry were conducted. LJ04 had a low EC50, high CC50 and high SI. LJ04 inhibited not only JN200804, but also JN200803 in RD cells, and viral proliferation was strongly inhibited, whereas LJ04 suppressed viral-induced apoptosis as detected by flow cytometry. In conclusion, LJ04 was found to have robust antiviral activity by inhibiting apoptosis and inducing IFN-beta expression. Our findings indicate that LJ04 is a good candidate for the treatment of EV71.

• 出版日期2017-12
• 单位山东省医学科学院; 聊城市人民医院; 山东第一医科大学