Down-Regulation of MiR-30c Promotes the Invasion of Non-Small Cell Lung Cancer by Targeting MTA1

作者：Xia, Yang; Chen, Qiyou; Zhong, Zhaopeng; Xu, Caihua; Wu, Chen; Liu, Bin; Chen, Yijiang^*

来源：Cellular Physiology and Biochemistry, 2013, 32(2): 476-485.

DOI：10.1159/000354452

• MicroRNA-30c
• MTA1
• Invasion
• NSCLC

Background: The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC). Methods and Results: Comparing with adjacent tissues (n = 75), miR-30c has a lower expression in lung cancer specimens (n = 75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3'-untranslated regions (3'-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c. Conclusion: miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC.

• 出版日期2013
• 单位南京医科大学