科研之友
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摘要
Context: Baccharis psiadioides (Less.) Joch. Muller (Asteraceae) is considered as a producer of bioactive essential oils and is used in south Brazilian folk medicine for its proprieties as stimulant, antipyretic, anti-inflammatory and as an antidote for snake bites. Objective: To verify the antimicrobial and antibiofilm activities of the essential oil of B. psiadioides (EOBP) against antibiotic-resistant Enterococcus faecalis. Materials and methods: The initial evaluation of EOBP activity was conducted by the agar and microdilution methods against 13 antibiotic-resistant E. faecalis strains. The antibiofilm effect was determined by the application of EOBP in the earlier adherent cells or to the stabilized biofilm for 24 h and was evaluated by crystal violet, viability and scanning electron microscopy (SEM) assays. Chemical composition of EOBP was determined by gas chromatography (GC/FID - GC/MS). Results: The MIC values for EOBP were at least 1.25% and 4-16% for agar and microdilution assays, respectively. The EOBP reduced the microbial adherence and the viability of the cells, but did not cause the complete disruption of biofilms. SEM images indicate that EOBP influences the adherence of cells to a surface. The monoterpene beta-pinene was the major constituent identified in EOBP. Conclusion: This research shows the ability of EOBP to control resistant E. faecalis strains and to reduce the biofilm amount attached to abiotic surfaces, indicating its role as a promising new natural antimicrobial agent. Moreover, these results further contribute to the growing number of studies of plant natural products which suggest that these compounds can combat resistant microorganisms.
- 出版日期2016-12
