科研之友
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摘要
The toxicity of inhalable mineral granules has become a great interest topic of air pollution in the international. The majority of research in this area has focused on organic components that adhere to inhalable granules and on the pathogenicity of granules-associated heavy metal ions. However, as the main components of the mineral grains of dust, inhalable mineral granules is an important part of the dust, the granules themselves also represent important pathogenic factors. Whether the inhalable mineral granule in China has the genetic toxicity is unknown. To determine whether the mineral granules are genotoxic, a specific concentration range for 6 major components of the inhalable minerals in China were selected. The human lung adenocarcinoma cell line A(549) was used to study granules genetic toxicity. Wright-Giemsa staining was used to evaluate cell morphology, the methyl thiazolyl tetrazolium (MTT) assay was used to determine any effects on cell proliferation, micronucleus tests were conducted to observe the effects of the minerals on the formation of chromosomal breaks and sister chromosome exchange (SCE) experiments were used to assess whether DNA synthesis was affected. The experimental results indicated the following: The membrane destruction, nuclear pyknosis, and mineral surface adhesion were observed in A(549) cells treated with sericite or albite. In cells treated with quartz or montmorillonite, gaps between the cells were increased in size, the cells appeared more loosely arranged and became smaller than those of the control group, and a large amount of granules adsorbed to the cell surface. The inhibitory effect of the minerals tested on the proliferation of A(549) cells ranked as follows, from most to least inhibitory: nano-montmorillonite, nano-SiO2, nano-sericite, nano-quartz, albite, and nano-calcite. Mineral granules concentration correlated with an inhibition of the cell proliferation rate. As the concentration of the mineral granules increased, the incidence of micronuclei gradually increased for all minerals. All mineral granules tested caused DNA damage in the cells in a dose-dependent manner, as higher concentrations of mineral granules caused more DNA damage. These results indicate that inhalable mineral granules may be genotoxic to cells.
- 出版日期2016-1
- 单位西南科技大学
