A Toll-like receptor 3 agonist (polyI:C) elicits innate host responses in the spleen and lungs of chickens

作者:Parvizi Payvand; Mallick Amirul Islam; Haq Kamran; Schlegel Benjamin; Sharif Shayan*
来源:Canadian Journal of Veterinary Research, 2012, 76(3): 230-234.

摘要

Double-stranded (ds) RNA interacts with host Toll-like receptor (TLR-3), leading to the induction of anti-viral host responses. The present study was designed to compare different routes of administration of a synthetic dsRNA (polyI: C) for induction of innate responses in chicken spleen and lungs. Chickens were treated with polyI: C via the aerosol, intra-air sac (i.a.s.), and intramuscular (IM) routes. Spleen and lungs were collected at 0, 2, 6, 12, and 24 h post-treatment and the expression of innate defence genes was quantified by real-time reverse transcriptase polymerase chain reaction (RT-PCR). There was an up-regulation of interferon (IFN)-beta, TLR-3, and Toll/interleukin 1 receptor domain-containing adaptor protein inducing IFN-beta (TRIF) at 6 h post-treatment in the spleen via IM administration of polyI: C. There was also an increase in the expression of TLR-3 and TRIF in the spleen at 2 h post-treatment via the i.a.s. route. The expression of IFN-alpha and TRIF was upregulated at 6 h post-treatment via the i.a.s. route in the lungs. Overall, our results indicate that administration of polyI: C can locally and systemically induce the expression of innate response genes depending on the route.

  • 出版日期2012-7