Molecular analyses of HLA-DRB1,-DPB1, and -DQB1 in Jing ethnic minority of Southwest China

作者：Lin, JH; Liu, ZH; Lv, FJ; Fu, YG; Fan, XL; Li, SY; Lu, JM; Liu, XY; Xu, AL^*

来源：Human Immunology, 2003, 64(8): 830-834.

DOI：10.1016/S0198-8859(03)00128-9

• HLA class II genes
• allele frequencies
• PCR-SBT
• Jing ethnic minority
• haplotype frequencies

In the present study, DNA typing for HLA-DRB1, DQB1 and DPB1 was performed using polymerase chain reaction-sequencing based typing (PCR-SBT) method in 144 random selected Jing ethnic individuals inhabiting in South China. Allele frequencies and two-locus haplotypes (DRB1-DQB1) were statistically analyzed and 20 DPB1 alleles, 27 DRB1 and 20 DQB1 were detected. The most frequent DPB1 allele was DPB1*0501 with the percentage of 36.996 followed by DPB1*1301 (15.7176), DPB1*0401 (11.0%) and DPB1*020102 (9.8%). Among the 27 detected DRB1 alleles, DRB1*120201 (13.8%) was most commonly observed followed by DRB1*150201, *030101 and *090102 alleles with the frequencies of 9.4176, 9.1% and 8.3%, respectively. Among the 20 detected DQB1 alleles the most predominant one was DQB1*030101/0309 (19.9176). DQB1*050201 (19.1%), DQB1*0201/0202 (16.196) and DQB1*050101 (12.3%) were also frequently observed in Jing population. Statistical analysis of two-locus haplotypes showed that DRB1*120201DQB1*030101/DRB1*120201-DQB1*0309 (HF = 9.4%, D = 6.65 x 10(-2)) was most predominant followed by DRB1*030101-DQB1*0201/DRB1*030101-DQB1*0202 (HF = 8.1176, D = 6.66 x 10(-2)). The comparison of HLA class II allele and haplotype frequencies in Jing with those in other populations all over the world and a dendrogram based on the DRB1, DQB1 and DPB1 genes suggested that Jing ethnic population has an origin of Southeast Asia and is belonged to the southern group of Chinese populations.

• 出版日期2003-8
• 单位中山大学; 中国疾病预防控制中心