Anti-Mycobacterium tuberculosis activity and cytotoxicity of Calophyllum brasiliense Cambess (Clusiaceae)

作者：Agostinho Pires Claudia Terencio; Brenzan Mislaine Adriana; de Lima Scodro Regiane Bertin; Garcia Cortez Diogenes Aparicio; Ghiraldi Lopes Luciana Dias; Dias Siqueira Vera Lucia; Cardoso Rosilene Fressatti^*

来源：Memorias do Instituto Oswaldo Cruz, 2014, 109(3): 324-329.

DOI：10.1590/0074-0276130323

• Mycobacterium tuberculosis
• Calophyllum brasiliense
• coumarin
• cytotoxicity
• 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide

We evaluated the in vitro anti-Mycobacterium tuberculosis activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of Calophyllum brasiliense. Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5- diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 +/- 2.6 and 8.24 +/- 1.1 mu g/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-M. tuberculosis H(37)Rv activity of 62.5 and 125 mu g/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 mu g/mL against M. tuberculosis H(37)Rv and clinical isolates. Compound (3) showed no activity against M. tuberculosis H(37)Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of C. brasiliense against M. tuberculosis.

• 出版日期2014-5