Mutation in clp(xoo4158) Reduces Virulence and Resistance to Oxidative Stress in Xanthomonas oryzae pv. oryzae KACC10859

作者：Cho Jung Hee; Jeong Kyu Sik; Han Jong Woo; Kim Woo Jae; Cha Jae Soon^*

来源：Plant Pathology Journal, 2011, 27(1): 89-92.

DOI：10.5423/PPJ.2011.27.1.089

• bacterial blight
• Clp
• oxidative stress
• rpf gene
• Xanthomonas oryze pv. oryzae

Cyclic AMP receptor-like protein (Clp), is known to be a global transcriptional regulator for the expression of virulence factors in Xanthomonas campestris pv. campestris (Xcc). Sequence analysis showed that Xanthomonas oryzae pv. oryzae (Xoo) contains a gene that is strongly homologous to the Xcc clp. In order to determine the role of the Clp homolog in Xoo, a marker exchange mutant of clp,, was generated. Virulence and virulence factors, such as the production of cellulase, xylanase, and extracellular polysaccharides (EPS) and swarming motility were significantly decreased in the clp(xoo4158) mutant. Moreover, the mutation caused the strain to be more sensitive to hydrogen peroxide and to over-produce siderophores. Complementation of the mutant restored the mutation-related phenotypes. Expression of assessed by reverse-transcription real-time PCR and clp promoter activity, was significantly reduced in the rpfB, rpfF, rpfC, and rpfG mutants. These results suggest that the clp homolog, clp(xoo4158), is involved in the control of virulence and resistance against oxidative stress, and that expression of the gene is controlled by RpfC and RpfG through a diffusible signal factor (DSF) signal in Xanthomonas oryzae pv. oryzae KACC10859.

• 出版日期2011-3