Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

作者：Nagashima F; Kondoh M; Kawase M; Simizu S; Osada H; Fujii M; Watanabe Y; Sato M; Asakawa Y^*

来源：Planta Medica, 2003, 69(4): 377-379.

DOI：10.1055/s-2003-38888

ent-11alpha-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

• 出版日期2003-4