科研之友
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摘要
In rodents, the preovulatory luteinizing hormone (LH) surge is timed by a circadian rhythm We recently reported that a phenobarbital-induced delay of the estrous cycle m Syrian hamsters is associated with an approximately 2-h phase advance in both the circadian locomotor activity rhythm and the timing of the LH surge The following study tests the hypothesis that a >2-h nonpharmacological phase advance in the circadian pacemaker that delays the estrous cycle by a day will also phase advance the LH surge by approximately 2 h Activity rhythms were continuously monitored in regularly cycling hamsters using running wheels or infrared detectors for about 10 days prior to jugular cannulation The next day, on proestrus, hamsters were transferred to the laboratory for 1 of 3 treatments transfer to a "new cage" (and wheel) from zeitgeber time (ZT) 4 to 8 (with ZT12 defined as time of lights-off), or exposure to a "novel wheel" at ZT5 or ZT1 All animals were then placed m constant dark (DD) Blood samples were obtained just before onset of DD and hourly for the next 6 h, on that day and the next day for determination of plasma LH concentrations Running activity was monitored in DD for about 10 more days Transfer to a novel wheel at either ZT5 or ZT1 delayed the LH surge to day 2 in most hamsters, whereas exposure to a new cage did not Only the delayed LH surges were phase advanced at least 25 h on average in all 3 groups However, wheel-running activity was similarly phase advanced in all 3 groups regardless of the timing of the LH surge, thus, the phase advances in circadian activity rhythms were not associated with the 1-day delay of the LH surge Interestingly, the number of wheel revolutions was closely associated with the 1-day delay of LH surges following exposure to a novel wheel at either ZT1 or ZT5 These results suggest that the intensity of wheel running (or an associated stimulus) plays an important role in the circadian timing mechanism for the LH surge
- 出版日期2010-12
