MAPPING OF MAREKS-DISEASE VIRUS GENOME - IDENTIFICATION OF JUNCTION SEQUENCES BETWEEN UNIQUE AND INVERTED REPEAT REGIONS

作者：MAKIMURA K^*; PENG FY; TSUJI M; HASEGAWA S; KAWAI Y; NONOYAMA M; TANAKA A

来源：Virus Genes, 1994, 8(1): 15-24.

DOI：10.1007/BF01703598

• MDV BAMHI MAPPING
• JUNCTION OF INVERTED REPEAT AND UNIQUE REGION
• PP38

Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TR(L)/U-L, U-L/IR(S), IR(S)/U-S, and US/TR(S). The TR(L)/UL junction is located 192 bp downstream of the last EcoRI site in the TR(L) region, while the U-L/IR(L) junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IR(L) region. The IR(S)/U-S junction is located 950 bp downstream of the second EcoRI site in the IR(S) region, while the U-S/TR(S), junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TR(S) region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U-2 and -P-4, upstream of the U-S/TR(S) junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TR(L)/U-L junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the U-L/IR(L) region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain.

• 出版日期1994-1