Absence of mutagenic and recombinagenic activity of multi-walled carbon nanotubes in the Drosophila wing-spot test and Allium cepa test

作者：de Andrade Laise Rodrigues; Brito Arian Sandin; Gouvea de Souza Melero Anna Maria; Zanin Hudson; Ceragioli Helder Jose; Baranauskas Vitor; Cunha Kenya Silva^*; Irazusta Silvia Pierre

来源：Ecotoxicology and Environmental Safety, 2014, 99: 92-97.

DOI：10.1016/j.ecoenv.2013.10.013

• Nanoparticles
• Recombination
• Mutation
• Genotoxicity

In order to assess the safety of the carbon nanotubes to human health and the environment, we investigated the potential toxicity and ability of multi-walled carbon nanotubes (NT), to induce DNA damage by employing the Allium cepa genotoxicity/mutagenicity test and the Somatic Mutation and Recombination Test (SMART) in the fruitfly, Drosophila melanogaster. The results demonstrated that NT did not significantly induce genotoxic or mutagenic effects in the Allium cepa test. All concentrations evaluated in the SMART assay showed survival rates higher than 90 percent, indicating the absence of chronic toxicity for NT. Furthermore, the various treatments showed no significant increase in the NT mutation and recombination frequencies in mwh/flr(3) genotype compared to respective negative controls, demonstrating the absence of DNA damage caused by NT.

• 出版日期2014-1