Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes

作者：Boczonadi Veronika; Maeaettae Arto

来源：FEBS Letters, 2012, 586(19): 3090-3096.

DOI：10.1016/j.febslet.2012.07.057

• Periplakin
• Annexin
• Epidermis
• Epithelia
• Co-immunoprecipitation
• Epidermal barrier

Periplakin regulates keratin organisation and participates in the assembly of epidermal cornified envelopes. A proteomic approach identified annexin A9 as a novel interacting partner for periplakin N-terminus. The presence of annexin A9 in complexes with periplakin was confirmed by immunoblotting of proteins immunoprecipitated by anti-HA or anti-annexin A9 antibodies. Both endogenous and GFP-tagged annexin A9 co-localise with endogenous periplakin and transfected periplakin N-terminus at MCF-7 cell borders and aggregate after Okadaic acid treatment. Annexin A9 and periplakin co-localise in the epidermis and annexin A9 is up-regulated in differentiating keratinocytes, but the epidermal annexin A9 expression does not require periplakin. %26lt;br%26gt;Structured summary of protein interactions: %26lt;br%26gt;Annexin-9 physically interacts with Periplakin by anti bait co-immunoprecipitation (View interaction) %26lt;br%26gt;Periplakin physically interacts with Annexin-9 by anti tag co-immunoprecipitation (View Interaction: 1,2) %26lt;br%26gt;Periplakin and Annexin-9 colocalize by fluorescence microscopy (View Interaction: 1, 2, 3

• 出版日期2012-9-21