RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-IV (B-G) GENOTYPES IN MEAT-TYPE CHICKENS

作者：UNI Z^*; HILLEL J; WAIMAN R; CAHANER A; HELLER DE

来源：Animal Genetics, 1992, 23(4): 379-384.

• CHICKEN
• RFLP
• MHC CLASS-IV

Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat-type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and Bgl II. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B-G genotypes in the meat type chickens.

• 出版日期1992